益气养阴加祛风药方对动脉粥样硬化大鼠腹主动脉内中膜厚度及炎症因子表达影响的实验研究
Experimental study on the effect of Yiqi Yangyin plus Qufeng Prescription on Immu-ne Inflammatory Factor and intraperitoneal aorta mesmembrane Thickness in Athero-sclerosis Rats
谢心 1徐蓓峥 2樊月月 1罗浩 1韩明珠3
作者信息
- 1. 上海市中西医结合医院内分泌科,上海 200082
- 2. 上海市虹口区凉城社区卫生服务中心,上海 200434
- 3. 临沂市郯城县第一人民医院内分泌科,山东临沂 276199
- 折叠
摘要
目的:本研究通过观察益气养阴加祛风药方对动脉粥样硬化大鼠治疗前后免疫炎症因子hs-CRP、TGF-β1、IL-8、MMP-9的表达水平及对腹主动脉内中膜厚度影响来观察益气养阴加祛风药方的实验疗效.方法:采用随机对照方法将46只SD大鼠随机分为四组:空白组、模型组、中药组和西药组.空白组予以基础饲料喂养,其余各组动物采用主动脉内膜球囊拉伤术加高脂饲料喂养完成动脉粥样硬化大鼠模型,中药组给予益气养阴加祛风药方,西药组给予瑞舒伐他汀片.给药6周后腹主动脉剪取粥样斑块留作标本,检测hs-CRP、TGF-β1、IL-8、MMP-9的表达水平及腹主动脉内中膜的厚度变化.结果:用SNK法两两比较,中药组与模型组、西药组与模型组在hs-CRP、TGF-β1、IL-8、MMP-9的表达水平均明显下降,两组比较均有显著性差异(P＜0.05),对腹主动脉内中膜厚度的变化也有统计学差异(P＜0.05).结论:益气养阴加祛风药方对动脉粥样硬化大鼠治疗前后hs-CRP、TGF-β1、IL-8、MMP-9的表达水平及腹主动脉内中膜厚度均有明显改善作用,为临床对动脉粥样硬化的干预治疗提供一个研究方向.
Abstract
Objective:To observe the effect of Yiqi Yangyin Qufeng Prescription on the expression level of immune inflam-matory factor hs-CRP、TGF-β1、IL-8、MMP-9and the change of intraperitoneal aorta mesmembrane thickness before and af-ter treatment in Ath-erosclerotic rats.Methods:46SD rats were randomly divided into four groups:blank group,model group,traditional Chinese medicine group and western medicine group.The blank group was fed with basic feed,the other groups were fed with aortic intimal balloon injury plus high fat feed,the traditional Chinese medicine group was given Yiqi Yangyin Qufeng Prescription and the western medicine group was given rosuvastatin tablets.After 4weeks of administration,the abdominal aorta was cut to retain the atherosclerotic plaque as a specimen,and the expression level of the hs-CRP、TGF-β1、IL-8、MMP-9and the thickness of intraperitoneal aorta mesmembrane were measured.Results:Compared with the SNK method,there were significant differences in the expression level of hs-CRP、TGF-β1、IL-8、MMP-9between Chinese medicine group and model group,western medicine group and model group(P＜0.05).but also statistically significant changes in the thickness of the medi-al membrane in intraperitoneal aorta mesmembrane(P＜0.05).between Chinese medicine group and model group,western medi-cine group and model group.Conclusion:Yiqi Yangyin plus Qifeng prescription can significantly improve the expression level of hs-CRP、TGF-β1、IL-8、MMP-9and the thickness of intraperitoneal aorta mesmembrane before and after treatment in athero-sclerotic rats.
关键词
益气养阴加祛风药方/动脉粥样硬化/炎症因子/腹主动脉内中膜Key words
Yiqi Yangyin plus/Qifeng prescription/Atherosclerotic inflammatory factor/Intraperitoneal aorta mesmembrane引用本文复制引用
基金项目
上海市海派中医流派传承人才培养项目(RCPY004)
上海市科委医学引导类科研项目(HKQ-ZYY-2020-32)
出版年
2024
国家科技期刊平台
NSTL
万方数据