首页|基于"脑肠轴理论"探讨龟羚帕安丸对PD大鼠纹状体DA及血清CCK、VIP水平影响的研究

基于"脑肠轴理论"探讨龟羚帕安丸对PD大鼠纹状体DA及血清CCK、VIP水平影响的研究

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目的:基于"脑肠轴理论"探讨龟羚帕安丸对PD大鼠纹状体裂解液多巴胺(Dopamine,DA)、血清胆囊收缩素(cholecystokinin,CCK)及血管活性肠肽(vasoactive intestinal peptide,VIP)水平的影响.方法:采用6-羟基多巴胺注射法制作PD大鼠模型,随机分为模型组、西药组、中药高、中、低剂量组,每组15只,另设15只假手术组.模型组及假手术组给予等容积生理盐水灌胃,其余组给予相应药物灌胃,连续给药4周.酶联免疫吸附测定法(ELISA)检测纹状体裂解液DA及血清CCK、VIP水平.结果:模型组大鼠纹状体DA、血清CCK及VIP水平显著降低;中药各剂量组、西药组大鼠纹状体DA、血清CCK及VIP水平明显均增加(P<0.01).结论:龟羚帕安丸具有明显神经保护作用,作用机制与激活脑肠轴、增加血清CCK及VIP水平、增加中脑黑质纹状体DA的水平、发挥改善肠道功能作用有关.
Based on the Theory of Brain-gut Axis,to Explore the Effects of Guiling Pa'an Pill on Striatal DA and Serum CCK and VIP Levels in PD Rats
Objective Based on the brain-gut axis theory,the effects of Guilingpa'an Pill on the levels of dopamine(Dopa-mine,DA)in striatum lysate and serum cholecystokinin(cholecystokinin,CCK)and vasoactive intestinal peptide(vasoactive intestinal peptide,VIP)in PD rats were studied.Methods The PD rat model was made by injection of 6-hydroxydopamine(6-OHDA).The successful PD rats were randomly divided into model group(n=15),western medicine group(n=15),high dose group(n=15),middle dose group(n=15)and low dose group(n=15).The model group and sham operation group were given the same volume of normal saline,and the other groups were given corresponding drugs for 4weeks.Blood samples were taken from abdominal aorta of rats,and the levels of DA in striatum lysate and serum CCK and VIP were detected by enzyme-linked im-munosorbent assay(ELISA).Results Compared with sham operation group,the levels of striatum DA,serum CCK and VIP in model group,western medicine group and each dose group were significantly lower than those in sham operation group(P<0.01);Compared with the model group,the levels of striatum DA,serum CCK and VIP in the western medicine group and each dose group were significantly higher than those in the model group(P<0.01).Conclusion Guiling Pa'an Pill has obvious neuro-protective effect,and its mechanism is related to activating brain-gut axis,increasing the level of CCK and VIP,increasing the level of DA in substantia nigra and striatum of midbrain,and improving intestinal function.

Parkinson's diseaseGuiling Pa'an pillDACCKVIP

常学辉、张良芝、陈帅杰、王冬莲、张创业

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河南中医药大学第二临床医学院,河南郑州 450002

河南中医药大学中医学院(仲景学院),河南郑州 450056

帕金森病 龟羚帕安丸 多巴胺 胆囊收缩素 血管活性肠肽

河南省科技攻关计划河南省中医药科学研究专项河南省中医药拔尖人才培养项目全国名老中医药专家传承工作室建设项目

2221023106302019ZYBJ16豫中医科教[2018]35号豫卫中医函20223号

2024

四川中医
四川省中医药学会,四川省中西医结合学会,四川省针灸学会,四川省中医药科学院

四川中医

CSTPCD
影响因子:0.522
ISSN:1000-3649
年,卷(期):2024.42(1)
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