Study on the Mechanism of Huanglian Jiedu Decoction on ERS Based on IRE 1α/XBP1/CHOP Pathway
Objective To study the effect of Huanglian Jiedu Decoction(HLJDD)containing serum on endoplasmic reticulum transmembrane kinase 1(IRE1)in the endoplasmic reticulum stress of human umbilical vein endothelial cells(HUVEC)in-duced by tunicamycin α、The effect of X box binding protein(XBP1)and cyclic adenosine monophosphate response element binding transcription factor homologous protein(C/EBP homologous protein,CHOP)on the expression of X box binding protein(XBP1)was studied to explore the cellular pathway mechanism of Huanglian Jiedu decoction on endoplasmic reticulum stress.Methods HLJDD and captopril containing serum were prepared,and the cells were divided into control group,TM group,HLJDD low,medium and high dose groups,and captopril group.The cell survival rate was detected by CCK8method;The level of apop-tosis was detected by flow cytometry.The protein expression of endoplasmic reticulum stress glucose-regulated protein 78kD(GRP78),CHOP,IRE1α,XBP1,PDIA6,eNOS was detected by protein blotting;and the mRNA of GRP78,CHOP,IRE1α,XBP1,PDIA6,eNOS was detected by real-time fluorescent quantitative PCR.Results Results Compared with blank control group,the cell survival rate in model group was decreased,the apoptosis rate was increased,and the expressions of GRP78,CHOP,IRE1α,XBP1and PDIA6were increased(P＜0.05).The expression of eNOS was decreased(P＜0.05).Com-pared with model group,the apoptosis rate and the expressions of GRP78,CHOP,IRE1α,XBP1and PDIA6were decreased sig-nificantly in Huanglian Jiedu decoction group and captopril group(P＜0.05).eNOS expression was increased(P＜0.05).Con-clusion Huanglian Jiedu Decoction can improve ERS status,inhibit cell apoptosis,and protect endothelial cells.The mechanism may be related to the inhibition of IRE1α/XBP1/CHOP signaling pathway.
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