Effect of Huazheng Huisheng Dan and its disassembled prescription on IL-4,IL-12and Tregs cells in Lewis Lung Cancer Model mice
Objective:To study the mechanism of anti-tumor immune micro-environment.Method:According to their body weight,112C57BL/6mice were randomly divided into three groups:Huazheng Huishengdan group,Huoxue Huayu group,Ruan-jian Sanjie group,qi analgesia group,tonifying qi and tonifying blood group,model group,cisplatin group and blank group with 12mice in each group.Except for the blank group,the lung cancer model of Lewis mice was established in the gluteal back of the other mice.Each traditional Chinese medicine group was given 0.4ml decoction(5.8g/kg)every day,the model group and blank group were given the same amount of saline every day,and the DDP injection was diluted to 0.002g·kg-1 in the platinum group,and 0.4ml was injected intraperitoneally in each mouse.After 14days,the mice were killed and the tumor tissue was taken to calculate the tumor inhibition rate.The expression of IL-4,IL-12was detected by ELISA method and the expression of CD4Tregs,CD4CD25Foxp3Tregs was detected by flow cytometry.Results:Compared with the model group,the levels of IL-4in peripheral blood of lung cancer mice in the whole prescription group,Huoxue Huayu group,Ruangjian Sanjie group and tonifying qi and tonifying blood circulation group were significantly lower than those in the model group(P＜0.05).Compared with the model group,Huazheng Huishengdan whole prescription group,Huoxue Huayu group The level of IL-12in peripheral blood of lung cancer mice in Xuehuayu group,Ruangjian Sanjie group and invigorating qi and tonifying blood group was significantly high-er than that in model group,and the differences in data were statistically significant(P＜0.05).Compared with the model group,Huazheng Huisheng Dan group,Huoxue Huayu group,Ruanjian Sanjie group and tonifying qi and tonifying blood group could sig-nificantly reduce the proportion of CD4CD25Foxp3Tregs/CD4Tregs cells in peripheral blood,the difference was statistically signif-icant(P＜0.05).Conclusion:Huazheng Huisheng Dan and its disassembled prescription may promote the secretion of IL-12by down-regulating the expression of IL-4.Increasing and decreasing the proportion of CD4CD25Foxp3Tregs cells has an effect on tumor immune microenvironment.
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