The effect of Modified Simiao Yong'an Decoction formula on THP-1 Macrophage-Derived Foam Cells
OBJECTIVE:To observe the effects of Modified Simiao Yong'an Decoction extracts on cytokine levels and foam cell numbers and morphology in THP-1 macrophage-derived foam cell models under different dosages and administration frequen-cies.METHODS:Successfully modeled THP-1 macrophage-derived foam cells were divided into the whole formula group and the refined group,and Modified Simiao Yong'an Decoction extract was added to the cell culture medium at a herb concentration equiv-alent to 0.1g·μL-1.The cells were stimulated for 48hours,and the supernatant was used to detect cytokine levels by ELISA,while the number and morphological changes of foam cells were observed.The whole formula group was given a daily dose of 1～10,12,and 16μl-d-1 for 12dose groups,while the refined group was given a single dose of 1,3,or 4µl three times daily for three dose groups.RESULT:In the whole formula group,except for the levels of IL-8and TNF-α,which exceeded the upper limit of detection,the levels of IL-1β and IL-10decreased rapidly with the increase of the dose less than 12μl-d-1,and the lev-els of other cytokines remained roughly stable,while the levels of IFN-α and IL-5decreased rapidly with the increase of the dose greater than 12μl-d-1,and the levels of IL-1β,IL-2,IL-6,IL-171evels increased rapidly.In terms of foam cell number,there was an increasing trend in the interval of less than 6μl-d-1 administration,a decreasing trend in the interval between 6～12µl-d-1,and a significant increase in foam cell number at 16μl-d-1 administration.When comparing between the whole formu-la group and the refined group,there were significant differences in IL-1β(at 3,9,and 12μl-d-1),IL-6(at 9μl-d-1),and IL-10(at 12µl-d-1)levels(P＜0.05),while there were no significant differences in the levels of other cytokines(P＞0.05).In terms of foam cell number,there were no significant differences between the groups at 3 μl-d-1 administration(P＞0.05),but there were significant differences between the groups at 9and 12µl-d-1 administration(P＜0.05).CONCLUSION:Modified Simiao Yong'an Decoction extract has a dose-response relationship,and both daily dose and administration frequency can affect foam cell number,morphology,and cytokine levels.Therefore,the dosage and administration frequency of traditional Chinese medicine are important factors affecting clinical efficacy,and changing the administration mode may be an effective way to improve clinical efficacy.
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