Effects of PI3K/Akt/NF-κB signaling pathway regulated by lozingin on myocardial injury in rats with coronary heart disease
Objective To investigate the effects of alpinogenin regulating phosphatidylinositol 3-kinase(PI3K)/protein ki-nase B(Akt)/nuclear transcription factor-κB(NF-κB)signaling pathway on myocardial injury in rats with coronary heart dis-ease(CHD).Methods 60healthy adult SD rats with SPF were randomly divided into blank group,model group,small dose group,medium dose group and high dose group with 12rats each.The PI3K/Akt/NF-KB signaling pathway protein expression was detected by Westem blot assay.Inflammatory factors[tumor necrosis factor α(TNF-α),interleukin-1 β(IL-1β),interleukin-18(IL-18)]and oxidative stress[reactive oxygen species(ROS),glutathione peroxidase(GSH-Px),malondialdehyde(MDA)]were detected by ELISA.The myocardial injury of rats was observed and recorded.Results Compared with the blank group,the area of myocardial ischemia and myocardial infarction in the model group was larger(P＜0.05).Compared with the model group,the area of myocardial ischemia and myocardial infarction in the small,medium and high dose groups of lozingin de-creased(P＜0.05),and the area of myocardial ischemia and myocardial infarction in the high dose group was smaller than that in the medium and small dose groups of lozingin.The area of myocardial ischemia and myocardial infarction in medium dose group was smaller than that in small dose group(P＜0.05).Compared with blank group,PI3K,Akt and NF-κB in model group were higher(P＜0.05),and PI3K,Akt and NF-κB in small,medium and high dose groups were increased compared with model group(P＜0.05),and PI3K,Akt and NF-κB in high dose group were higher than those in medium and low dose groups.PI3K,Akt and NF-κB in medium dose group were higher than those in small dose group(P＜0.05).Compared with the blank group,the levels of TNF-α,IL-1 β and IL-18in the model group were higher(P＜0.05),and the levels of TNF-α,IL-1β and IL-18in the small,medium and high dose groups were lower than those in the model group(P＜0.05).The levels of TNF-α,IL-1βand IL-18in high-dose group were lower than those in medium-dose and low-dose groups,and the levels of TNF-α,IL-1β and IL-18in medium-dose group were lower than those in low-dose group(P＜0.05).Compared with blank group,ROS,GSH-Px and MDA in model group were higher(P＜0.05).Compared with model group,ROS,GSH-Px and MDA in small,medium and high dose groups were decreased(P＜0.05),and ROS,GSH-Px and MDA in high dose group were lower than those in medium and small dose groups.The ROS,GSH-Px and MDA of the medium dose group were lower than those of the small dose group(P＜0.05).Conclusion Argentin can improve myocardial inflammation and oxidative stress in CHD rats by regulating PI3K/Akt/NF-κB signaling pathway,and thus play a protective role in myocardium.
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