Protective effect of total flavonoids of Engelhardtia roxburghiana on myocardial ischemia-reperfusion injury in rats with heart failure
Objective:To investigate the protective effect of total flavonoids of Engelhardtia roxburghiana(TFER)on myo-cardial ischemia-reperfusion injury(IRI)in rats with heart failure(HF).Methods:After successful modeling,60male SD rats were randomly divided into 5groups:the sham operation group,the IRI model group,the low-dose TFER group,the medium-dose TFER group,and the high-dose TFER group,with 12rats in each group.They were given 7d of continuous gavage.Electro-cardiography and echocardiography were used to detect S-T amplitude,left ventricular fractional shortening(LVFS),left ventric-ular ejection fraction(LVEF),maximum rate of rise of left ventricular pressure(+dp/dtmax)and maximum rate of decrease in left ventricular pressure(-dp/dtmax).Hematoxylin-eosin(HE)staining was used to detect the percentage of myocardial infarc-tion area in rats,and Masson staining was used to detect the percentage of collagen attachment area in myocardium.Colorimetric method was used to detect superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA).Western blot was used to detect the expression levels of nuclear factor E2related factor 2(Nrf2)and Kelch-like ECH-associated protein 1(Keap1).Results:Compared with the IRI model group,S-T segment on electrocardiogram of IRI rats treated with TFER gavage was repaired significantly.The percentage of myocardial infarction area,percentage of collagen attachment area,MDA and Keap1expression were significantly reduced.LVFS,LVEF,+dp/dtmax,-dp/dtmax,SOD,GSH Px and Nrf2expression were significantly increased(P＜0.05).Above-mentioned indices in the high-dose TFER group showed the most significant differences(P＜0.05).Conclusion:TFER have protective effect on IRI in rats with HF.The mechanism may be reducing myo-cardial infarction area,improving cardiac function,and alleviating oxidative stress response.
Total flavonoids of Engelhardtia roxburghianaHeart failureRatMyocardial ischemia-reperfusion injuryProtective effectMechanism
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维普
