摘要
目的:分析结直肠癌患者中医辨证分型与外周血微小RNA(miR)-21、白细胞介素(IL)-12、T淋巴细胞亚群水平变化及临床意义.方法:回顾性分析 2020 年 2 月～2023 年 8 月我院肿瘤科收治的 204 例结直肠癌患者的病历资料,根据患者中医证型分为湿热内蕴证58 例、脾虚气滞证41 例、脾肾两虚证39 例、气血两虚证37 例、瘀毒内阻证29 例.比较不同中医证型结直肠癌患者外周血miR-21、IL-12、T淋巴细胞亚群水平.采用Pearson相关性分析结直肠癌中医证型与外周血miR-21、IL-12、CD3+、CD3+CD4+T 的相关性.结果:5 种证型结直肠癌患者血清 miR-21、IL-12、CD3+、CD3+CD4+T比较,差异有统计学意义(P＜0.05).脾肾两虚证患者血清miR-21、CD3+、CD3+CD4+T水平均低于瘀毒内阻证、气血两虚证、脾虚气滞证、湿热内蕴证患者(P＜0.05),IL-12 水平均高于其他 4 种证型患者(P＜0.05).Pearson相关性分析结果显示,结直肠癌中医证型与miR-21、CD3+、CD3+CD4+T均呈正相关,与IL-12 呈负相关(P＜0.05).结论:结直肠癌患者中湿热内蕴证占比最高,其次为脾虚气滞证.不同中医证型患者外周血miR-21、IL-12、CD3+、CD3+CD4+T水平存在差异,可为结直肠癌的中医辨证分型及病情评估提供参考.
Abstract
Objective:To study TCM syndrome differentiation and peripheral blood microrna(miR-21),interleukin(IL)-12and T lymphocyte subsets in patients with colorectal cancer and its clinical significance.Method:The medical records of 204patients with colorectal cancer admitted to Oncology Department of our hospital from February 2020to August 2023were retrospec-tively analyzed.According to TCM syndrome types,the patients were divided into 58cases of dampness-heat syndrome,41cases of spleen deficiency qi stagnation syndrome,39cases of spleen and kidney deficiency syndrome,37cases of qi and blood deficiency syn-drome,and 29cases of internal blockage of blood stasis.To compare the levels of miR-21,IL-12and T lymphocyte subsets in periph-eral blood of colorectal cancer patients with different TCM syndrome types.Pearson correlation was used to analyze the correlation be-tween TCM syndrome types of colorectal cancer and peripheral blood miR-21,IL-12,CD3+,CD3+CD4+T.Results:There were significant differences in serum miR-21,IL-12,CD3+,CD3+CD4+T among 5types of colorectal cancer patients(P＜0.05).The se-rum levels of miR-21,CD3+,CD3+CD4+T in patients with spleen-kidney deficiency syndrome were lower than those in patients with internal blockage of stasis,deficiency of qi and blood,spleen deficiency qi stagnation syndrome,and internal dampness-heat syn-drome(P＜0.05),and the levels of IL-12were higher than those in patients with other 4types of syndrome(P＜0.05).The results of Pearson correlation analysis showed that the TCM syndrome type of colorectal cancer was positively correlated with miR-21,CD3+,CD3+CD4+T,and negatively correlated with IL-12(P＜0.05).Conclusion:Damp-heat syndrome is the highest in colorectal cancer patients,followed by spleen deficiency qi stagnation syndrome.The levels of miR-21,IL-12,CD3+,CD3+CD4+T in peripheral blood of patients with different TCM syndrome types are different,which can provide reference for TCM syndrome differentiation and disease evaluation of colorectal cancer.
维普
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