Objective:The experimental rats model of diabetic peripheral neuropathy(DPN)was established to study the effects of pestle treatment on serum inflammatory factors and sciatic neuropathologic morphology of DPN rats,and to explore the neuroprotec-tive mechanism of pestle treatment on DPN from the perspective of inflammation.Methods:Thirty-eight SD male rats were randomly divided into the normal group(n=11)and the model group(n=27).After successful modeling,the remaining 24rats in the model group were randomly divided into model control group(n=8),mecobalamin group(n=8)and clubbing group(n=8).The remaining 8rats in the normal group were normal control group.Normal control group and model control group were given normal diet and high-fat and high-sugar diet,respectively,without any intervention measures.On the basis of the model control group,the mecobalamin group was gavaged with mecobalamin tablets(0.25mg/kg)dissolved in distilled water daily for 4weeks.In the pestle group,on the basis of the intervention in the model control group,the Kuixing pen of pestle was used to tap Zhiyang eight array and Mingmen eight array in turn,and the Qiyao Huanyuan Chu was used to run the Heche Road of Mingmen to Changqiang section,once a day,for 30min each time.The intervention lasted for 4weeks.During the experiment to observe and record each rat's general condition,body weight and blood glucose were measured weekly during the intervention period.PWT was measured before and after the experiment.Af-ter the experiment,the morphological structure of sciatic nerve was observed and the serum levels of TNF-α,IL-1β and MCP-1were detected.Results:After intervention,the PWT of the pestle group and the mecobalamin group was significantly lower than that of the model control group(P＜0.05),sciatic nerve damage was significantly reduced,and the serum levels of TNF-α,IL-1β and MCP-1were significantly decreased(P＜0.05).Conclusion:The pestle therapy can obviously improve the pathological morphology of sciatic nerve,and significantly reduce the levels of serum inflammatory factors TNF-α,IL-1β and MCP-1.The results indicate that pestle therapy is effective in treating DPN and has anti-inflammatory effect.
维普
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