Study on the mechanism of ovarian reserve function of electroacupuncture at acupoints of liver meridian in DOR model rats based on"treatment from the liver"
Study on the mechanism of ovarian reserve function of electroacupuncture at acupoints of liver meridian in DOR model rats based on"treatment from the liver"
Objective:To observe the effect of electroacupuncture at acupoints of liver meridian on the level of sex hormones in rats with decreased ovarian reserve function(diminished ovarian reserve,DOR),and to explore the effect and mechanism of electroa-cupuncture at acupoints of liver meridian on the secretion of TGF-β1in the treatment of DOR by regulating the expression of Smad2and Smad3protein.Methods:32clean female SD rats were randomly divided into blank group(n=8),model group(n=8),liver meridi-an group(n=8)and non-acupoint group(n=8).The rat model of DOR was established by intraperitoneal injection of cyclophospha-mide(CTX).The liver meridian group was treated with electroacupuncture at"Ququan acupoint","Taichong acupoint","Jimai acu-point"and"Ligou acupoint",and the non-meridian non-acupoint group was treated with electroacupuncture at non-meridian and non-acupoints,once every other day,3times a week for 3weeks.After treatment,the general condition of rats was observed,the es-trous cycle of rats was observed by HE staining,the levels of estradiol(E2),follicle stimulating hormone(FSH),luteinizing hor-mone(LH)and anti-Mullerian hormone(AMH)in serum were detected by enzyme-linked immunosorbent assay(ELISA),and the protein expressions of TGF-β1,Smad2and Smad3in rat ovary were detected by western blot.Results:Compared with the blank group,the body weight of the model group was decreased,the behavior habit was abnormal,the estrous cycle was prolonged and disordered in varying degrees,the levels of E2and AMH were significantly decreased,the levels of FSH and LH were increased,and the protein ex-pression levels of TGF-β1,Smad2and Smad3were significantly decreased in the model group.Compared with the model group,the body weight,activity,estrous cycle regularity,serum E2and AMH levels increased,FSH and LH levels decreased,TGF-β1,Smad2and Smad3protein expression increased significantly in the liver meridian group.The abnormal performance,estrous cycle disor-der rate and estrous cycle disorder rate were alleviated in the non-acupoint group(P＜0.01).The contents of FSH and LH decreased.Compared with the non-acupoint group,the estrous cycle of rats in the liver meridian group was regular,the levels of E2and AMH were increased,the levels of FSH and LH were decreased,and the protein expressions of TGF-β1,Smad2and Smad3were strongly ex-pressed and significantly increased.Conclusion:Electroacupuncture at acupoints of liver meridian can improve the ovarian reserve function of DOR rats,and its mechanism may be related to the regulation of TGF-β1,Smad2and Smad3protein expression.
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