Effect of Yuxian Decoction on intestinal microecology and serum VIP,CCK,IL-17,IL-23and IL-10expression in rats with ulcerative colitis of damp-heat type in large intestine
Objective:To investigate the effects of Yuxian Decoction on the intestinal microecology and the expression of VIP,CCK,IL-17,IL-23,IL-10in serum of rats with large intestine damp heat type ulcerative colitis to explore its possible mechanism of action of colitis.Methods:Forty-eight healthy Sprague-Dawley rats were randomly divided into six groups(blank control group,mod-el group,low dose treatment group,medium dose treatment group,high dose treatment group and Salazosulfadimidine group,eight rats in each group).The blank control group did not model,the rats in model group were used to prepare a rat model of ulcerative coli-tis by trinitro benzenesulfonic acid(TNBS).After successful modeling,the blank control group,the model group were given an equal dose of physiological saline,and each administration group was given low dose,medium dose and high dose Yuxian decoction and the sulfasalazine suspension rectally continuous rectally for 14d.The intestinal microecology in each group was observed by microflora cul-ture,including Bifidobacterium,Lactobacillus,Enterococcus and Enterobacter.The levels of acetic acid,propionic acid and butyric acid in intestinal tract of colon of mice in each group were detected by gas chromatography method.The contents of serum brain-gut peptide such as VIP,CCK,IL-17,IL-23,IL-10were detected by ELISA.Results:Compared with the blank control group,the contents of Bifidobacterium,Lactobacillus,acetic acid,propionic acid,VIP,CCK,IL-10were reduced in the model group,SASP group and each dose groups of Yuxian Decoction,and the level of IL-17,IL-23were increased,after treating with Yuxian Decoction,with the gradual increase of the administered dose,the contents of Bifidobacterium,Lactobacillus,acetic acid,propionic acid,VIP,CCK,IL-10was significantly increased(P＜0.05),and the IL-17,IL-23level was significantly reduced(P＜0.05).Conclusion:Yuxian Decoction shows good regulation on the intestinal microecology and short-chain fatty acids and brain-gut peptide in large intes-tine damp heat type ulcerative colitis.
万方数据
维普
