Tomato callus induction and establishment of genetic transformation system
Tomato is one of the commonly used model plants in the field of plant research.The efficient and stable genetic transformation system of tomato serves as the foundation for studying the functional biol-ogy of tomato genes.However,conventional genetic transformation of tomato is time-consuming.Using tomato callus tissue as the infestation target and finally obtaining the transgenic tomato callus tissue can greatly shorten the time required to obtain transgenic tomato materials.In this study,the tomato Micro-Tom(Solanum lycopersicum cv.Micro-Tom)was used as the experimental material to explore the induc-tion conditions for tomato callus and to establish a standardized genetic transformation system for tomato callus tissue.The results show that the primary root as the explant,a hormone ratio of 2,4-D 1 mg/L+6-BA 1 mg/L,a sucrose concentration of 30 g/L,and a light intensity of 3 000 lx are the optimal induc-tion conditions.The standard genetic transformation system for tomato callus includes callus culture,es-tablishment of suspension cells,agrobacterium infection,co-cultivation,selection of resistant callus,and nucleic acid identification.The above experimental results can serve as method references and provide scientific research materials for molecular mechanistic studies on tomatoes,as well as related experiments involving immunoprecipitation,high-throughput sequencing,and subcellular localization.
万方数据
维普
