Imaging and Tracing of Fusarium Moniliforme based on Quantum Dot Labeling Technology
Silicon carbide quantum dots were prepared by the chemical etching process developed as a new labeling material,successfully achieved its labeling of Fusarium moniliforme.Based on this labeling technology,the influence of phenolic acids on the growth and division status of the fungus was studied,and the infection mechanism of the fungus on Arabidopsis seedlings in continuous cropping gardens was preliminarily explored.The results showed that the diameter of the quantum dots prepared by the corrosion method is about 5 nm,which approximately spherical in shape.The maximum intensity of photoluminescence occurs when the excitation wavelength is 340nm.The surface of the quantum dots are unmodified,hydroxyl,carboxyl,and sulfydryl groups have already formed;under the stress of benzoic acid,Fusarium moniliforme showed rapid division,abundant spore production,and strong spore infection toxicity,and this trend was positively correlated with benzoic acid content;The dynamic tracing results indicate that the first site of infection by Fusarium moniliforme on Arabidopsis was the root hair zone,followed by epidermal cells.Finally,the fungus breaks through the last safety barrier cell wall of the plant and enters the nucleus to complete the entire infection process.
SiC quantum dotsfusarium moniliformefluorescence labelinglong-term imagingdynamic tracing
NETL
NSTL
万方数据
