Regulation of F3'5'H on Anthocyanin Biosynthesis in Potato Tubers
To explore the regulation mechanism of anthocyanin biosynthesis in potato tuber, F3'5'H was cloned and its sequence was analyzed, moreover the transcription of related genes in anthocyanin metabolic pathway was analyzed through RT-PCR and qRT-PCR, using purple potato and its red mutant as the materials. The results show that there is a significant decrease in the expression level of F3'5'H in red mutant, inidicating that F3'5'H plays a key regulatory role in the transformation from purple anthocyanin to red anthocyanin in potato. The purple potato and its red mutant possess the same coding sequence and promoter sequence of F3'5'H, suggesting that F3'5'H regulats the anthocyanin transformation on the transcriptional level. There is a transcription factor MYB binding site in F3'5'H promoter region. The MYB expression level in the red mutant is higher than that in the wild type, and MYC and WD40 encoding transcription factors also show the same tendency. Three transcription factors regulate the direction of anthocyanin biosynthesis from purple to red through inhibiting the expression of F3'5'H.
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