Starting of Completely Autotrophic Nitrogen Removal over Nitrite Process and Evolution of Microbial Community Structure in the Presence of Organic Matter
To promote the practical application of the completely autotrophic nitrogen removal over nitrite(CANON)process in treatment of organic-containing wastewater,starting,operating conditions and microbial community structure of CANON process in the presence of organic matter were investigated.Through the control strategy of low dissolved oxygen and biofilm bacteria enrichment,the inhibition of organic matter on anaerobic ammonia oxidizing bacteria(AnAOB)was alleviated,and starting of CANON process and enrichment of functional microorganisms were realized.The results show that,when the influent mass concentration of chemical oxygen demand in the influent is 600 mg/L,CANON process starts,and the highest total inorganic nitrogen removal rate is 79.46% during the stable operation of the reactor.Three-dimensional fluorescence spectra show that humic and fulvic acids are the main microbial metabolites.Microbial high-throughput sequencing results show that the relative abundance of Candidatus Kuenenia and Candidatus Brocadia,key genera of AnAOB,increase from 0.02% and 0.01% to 10.47% and 4.45% respectively,indicating that the enrichment of AnAOB is achieved successfully.
completely autotrophic nitrogen removal over nitriteorganic mattermicrobialthree-dimensional fluores-cence spectrumhigh-throughput sequencing
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