磁场对短程硝化微生物群落结构和酶活性的影响
Effects of Magnetic Field on Structure and Enzyme Activity of Short-range Nitrification Microbial Community
赵校宇 1张凤军 2孙绍钧 3孙绍芳 1王嘉斌1
作者信息
- 1. 济南大学 土木建筑学院,山东 济南 250022
- 2. 山东方云软件技术有限公司,山东 济南 250100
- 3. 青岛市市政工程设计研究院有限责任公司,山东 青岛 266061
- 折叠
摘要
为了探明磁感应强度对短程硝化系统的影响机制,构建磁性序批式活性污泥法反应器,对比磁感应强度对短程硝化过程氮转化的影响规律,并通过高通量测序等分子生物学手段研究磁场对微生物群落结构和酶活性的影响.结果表明:磁场对短程硝化过程有显著影响,在磁感应强度为 10、15 mT的磁场作用下,氨氮去除率低于无磁场对照组的;在磁感应强度为 5、10 mT的磁场作用下,所构建反应器的亚硝态氮累积率高于无磁场对照组的;磁场对所构建反应器中微生物群落结构和酶基因相对含量产生影响,当磁感应强度为5 mT时,OLB8 菌群、亚硝酸盐氧化酶的相对丰度减小,氨单加氧酶和羟胺脱氢酶的相对丰度增大,亚硝态氮累积效果更优.
Abstract
To explore the influence mechanism of magnetic induction intensity on the short-range nitrification system,magnetic sequencing batch activated sludge method short-range nitrification reactors were constructed,effect laws of mag-netic induction intensity on nitrogen conversion in short-range nitrification process were compared,and effects of magnetic field on structure and enzyme activity of microbial community was researched by using molecular biology technology such as high-throughput sequencing.The results show that the magnetic field has significant effects on the short-range nitrifica-tion process,under the action of magnetic field with the magnetic induction intensity of 10 mT and 15 mT,the removal rate of ammonia nitrogen is lower than that of the control group without magnetic field.Under the action of magnetic field with the magnetic induction intensity of 5 mT and 10 mT,the nitrous nitrogen accumulative rate of the constructed reac-tors is higher than that of the control group without magnetic field.The magnetic field affects the microbial community structure and the relative content of enzyme genes in the constructed reactors.When the magnetic induction intensity is 5 mT,the relative abundances of OLB8 flora and nitrite oxidase decrease,the relative abundances of ammonia monooxy-genase and hydroxylamine dehydrogenase increase,and the nitrous nitrogen accumulation effect is better.
关键词
磁场/短程硝化/微生物群落/酶活性/影响机制Key words
magnetic field/short-range nitrification/microbial community/enzyme activity/influence mechanism引用本文复制引用
基金项目
国家自然科学基金项目(52100088)
济南大学科技项目(XBS2001)
出版年
2024
NETL
NSTL
万方数据