山东省肉鸭粪污腐熟剂菌群调查
Investigation of the Fungal Community in Poultry Litter Fermentation Agent in Shandong Province
赵晓雨 1李明珠 2薛飞 2汤瑞发 3刘文哲 4李有志 5汤文利5
作者信息
- 1. 山东省饲料兽药质量检验中心,山东 济南 250100
- 2. 华沃德源环境技术(济南)有限公司,山东 济南 250600
- 3. 山东中新食品集团有限公司,山东 济南 250101
- 4. 新泰市天信农牧发展有限公司,山东 新泰 271212
- 5. 山东省饲料兽药质量检验中心,山东 济南 250100;山东省蛋鸡技术创新中心,山东 济南 250102
- 折叠
摘要
为调查山东省肉鸭粪污腐熟剂菌群的情况,本研究从德州、日照、菏泽、泰安等地区肉鸭养殖场收集8种在用肉鸭粪污腐熟剂,利用平板划线法将肉鸭粪污腐熟剂接种至营养琼脂培养基、YPD琼脂培养基、高氏合成一号琼脂培养基和MRS琼脂培养基上分离纯化,通过16S rDNA序列分析鉴定菌株.结果显示:8种在用肉鸭粪污腐熟剂中,62.5%为复合菌剂,菌群组合多为芽孢杆菌、假单胞菌和乳杆菌,菌种数量一般为106～107 CFU/g;37.5%为单一菌种,以枯草芽孢杆菌为主,菌种数量大约为1011 CFU/g.本研究通过调查了解山东省部分地区肉鸭粪污腐熟剂中菌群结构,为肉鸭粪腐熟剂菌群构建提供理论基础.
Abstract
This study aimed to investigate the microbial community structure of composting agents utilized in poultry manure management within Shandong Province.Eight different composting agents from biological duck farms located in Dezhou,Rizhao,Heze,Taian,and other regions were collected for analysis.These agents were inoculated onto nutrient agar,YPD agar,High One Synthesis Agar,and MRS agar using a plate streaking method for separation and purification.The bacterial strains were subsequently identified through 16S rDNA sequence analysis.Results indicated that among the eight composting agents available on the market in Shandong Province,62.5%were composite formulations predominantly consisting of Bacillus sporothermodurans,Pseudomonas sp.,and Lactobacillus sp.,with strain counts typically ranging from 106 to 107 CFU/g;conversely,approximately 37.5%represented single-strain formulations primarily featuring Bacillus sporothermodurans at concentrations around 1011 CFU/g.This investigation elucidates the microbial community structure present in certain poultry manure composting agents within Shandong Province and provides a theoretical foundation for future development of these microbial communities.
关键词
肉鸭粪污腐熟剂/微生物/菌群结构Key words
Poultry manure composting agents/Microorganisms/Microbial community structure引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据