Isolation,Identification and Drug Screening of a Strain of Mycoplasma Synoviae
To investigate the pathogen causing leg paralysis and joint swelling in parental breeder chickens and develop effective treatment strategies,sick chickens exhibiting lameness and paralysis symptoms were collected from a broiler breeder farm in Shandong Province.The Mycoplasma synoviae(MS)was successfully isolated and purified using modified Frey's medium.Subsequently,the 16S rRNA gene sequence of MS was amplified using PCR technology and subjected to sequence alignment.Additionally,the isolated strain was identified using an MS wild-type strain PCR detection kit.Finally,the minimum inhibitory concentrations(MICs)of various antibiotics against the isolated strain were determined,and a clinical evaluation of the drugs was conducted.The results showed that a strain of MS,named MS-17103,was successfully isolated and identified from sick chickens with lameness and paralysis symptoms collected from a meat breeder chicken farm in Shandong Province.This strain exhibited typical mycoplasma colony morphology and was confirmed as MS wild-type strain-positive by PCR.The drug sensitivity test revealed that MS-17103 was sensitive to valnemulin hydrochloride(MIC of 0.00076 mg/L)and tylosin tartrate(MIC of 0.00153 mg/L).The application of these two drugs in breeder chicken farms previously infected with MS showed a significant reduction in the positive rate of MS infection in the chicken population.This study provides an important reference for the subsequent development of treatment plans for this disease.
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