OPTIMIZATION OF CELL- WALL DEGRADING ENZYMES PRODUCTION CONDITION BY RHIZOCTONIA CEREALIS
In this experiment, orthogonal test was used to optimize the cell - wall degrading enzymes production condition. Rhizoctonia cerealis were induced and cultured by improving Marcus liquid medium. The optimal environmental conditions including cultivation time, pH and cultivation temperature were further investigated through orthogonal experiment in this paper. The results showed that the optimal conditions of cellulose production were 25℃, pH6.8 and cultivation for 7 days, the optimal conditions of pectinase production were 25℃, pH5.0 and 5 days, the optimal conditions of xylanase production were 25℃, pH6.0 and cultivation for 5 days.
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