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低温胁迫对两种樟树抗寒性生理的影响

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以猴樟和芳樟叶片为试材,通过低温处理研究了两种树木叶片MDA、脯氨酸、可溶性蛋白质及SOD活性和电导率的变化。结果表明:随着处理温度的降低,两种樟树的电解质外渗率呈“S”型上升;两种樟树的SOD活性及脯氨酸含量呈现先升后降的变化;可溶性糖、可溶性蛋白质及MDA含量则均呈现先降后升再降的变化;采用Fuzzy数学隶属函数综合评判法对猴樟和芳樟的抗寒性进行综合评判猴樟的抗寒性大于芳樟,这与利用电解质外渗率结合Logistic方程得出猴樟和芳樟的半致死温度(LT50)分别为-12.43、-10.71℃结果相一致;以猴樟为例,对低温胁迫过程中各抗寒指标隶属函数值进行的综合评判结果显示其抗寒性分抗寒性减弱期、增强期及减弱期。
The Effect of Cold Stress on the Cold Resistance Physiology of Two Camphor Species
Taking leaves of Cinnamonum bodinieri Levl and C. camphora L. (linalool-type) treated by cold temperature as test materials, the changes of MDA, Proline, Soluble protein and SOD activity, electrical conductivity in them were studied. The results indicated that for all the materials, with the decrease of temperature, the ion leakage percentage of the two species increased in S-shape, the SOD and POD activities decreased after an initial increase. The content of soluble protein, MDA and soluble sugar began to drop, then to increase and at last to drop again. By using fuzzy mathematics method, the cold resistance of two camphor tree species was evaluated as Cinnamonum bodinieri Levl>C. camphora L. (linalool-type), the semi-lethal temperature (LT50) of two camphor tree species calculated by the Logistic equation of ion leakage percentage was-12.43,-10.71℃, respectively. At last, by using a fuzzy mathematics method, the cold resistance of C. bodinieri Levl was divided into three periods, namely, the reducing stage, the enhancement setting stage, and the reducing stage.

Camphor treecold resistanceSOD activitycomprehensive evaluation

王宁、袁美丽、丁博

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河南科技大学 林学院,河南 洛阳 471000

洛阳市隋唐城遗址植物园,河南 洛阳 471002

樟树 抗寒性 SOD活性 综合评价

河南省教育厅高等学校重点科研项目河南科技大学青年科学基金

16A2200012013QN018

2016

山东农业大学学报(自然科学版)
山东农业大学

山东农业大学学报(自然科学版)

CSTPCDCSCD
影响因子:0.565
ISSN:1000-2324
年,卷(期):2016.(1)
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