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山东高优苹果园土壤细菌多样性特征分析

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探索高优苹果园根际土壤菌群特点,为改善果园土肥环境进而提高苹果产量和品质提供参考。采用16SrRNA高通量测序技术,分析比较了沂水、蒙阴及曲阜三地高优/低产苹果园根际土壤样品中细菌菌群多样性及果实品质。结果表明,高优果园的产量及单果质量、可溶性固形物含量及糖酸比等果品品质综合指标均明显优于其低产果园的。高优果园的土壤细菌种群数量、群落构成等明显优于其低产果园土壤的,其中,放线菌门及放线菌纲、绿弯菌门等相对丰度均降低,而变形菌门、酸杆菌门以及α-变形菌纲、γ-变形菌纲的相对丰度增高。三地高优果园相比,曲阜和沂水果园苹果果实的可溶性固形物含量和糖酸比均显著高于蒙阴果园,而蒙阴果园产量及果实果形指数明显大于沂水和曲阜果园。沂水和曲阜果园土壤中的细菌优势种群数量和均匀度均明显高于蒙阴果园,沂水和曲阜高优果园根际土壤细菌相对丰度与土壤酶活性和果实品质呈正相关。总之,高优果园土壤中菌落种群和构成更为优化稳定,细菌种群更加丰富,促进根吸收养分能力增强,果实品质和产量提高。
Role of Soil Microbial in Fuji Apple Orchards with High-yield and Good-quality in Shandong Province
The characteristics of rhizosphere soil flora in high quality apple orchards were studied to provide references for improving soil and fertilizer environment and improving apple yield and quality.We used 16SrRNA high-throughput sequencing technology to analyze and compare the bacterial flora diversity and fruit quality in rhizosphere soil samples of high-excellent/low-yield apple orchards in Yishui,Mengyin and Qufu.The results showed the yield and single fruit quality,soluble solid content and sugar-acid ratio of high-quality orchards were obviously better than those of low-yield orchards.The quantity and community composition of rhizosphere soil bacteria in high-quality orchards were obviously better than those in low-yield orchards,and the relative abundance of actinomycetes,actinomycetes and Chloroflexi decreased,the relative abundance of proteobacteria,acidobacteria,α-proteobacteria and γ-proteobacteria increased.The soluble-solids content and sugar-acid ratio of apple fruits in Qufu and Yishui orchards were significantly higher than those in Mengyin orchards,while the yield and fruit shape index in Mengyin orchards were significantly higher than those in Yishui and Qufu orchards.The number and evenness of dominant bacterial populations of rhizosphere soils in Yishui and Qufu orchards were significantly higher than those in Mengyin orchards,and the relative abundance of rhizosphere soil bacteria was positively correlated with soil enzyme activity and fruit quality in Yishui and Qufu high-yield orchards.In the high-quality orchard soil,the community composition is more optimized and stable,the bacterial population is more abundant,the ability of nutrient absorption in root is enhanced,and the fruit quality and yield are improved.

Applefruit qualityrhizosphere soil microbial communityhigh-throughput sequencing

钱琛、范伟国、张玮玮、杨洪强

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山东农业大学园艺科学与工程学院, 山东 泰安 271018

苹果 果实品质 根际土壤菌群 高通量测序

国家重点研发计划项目课题国家自然科学基金项目

2019YFD100010331772251

2024

山东农业大学学报(自然科学版)
山东农业大学

山东农业大学学报(自然科学版)

CSTPCD北大核心
影响因子:0.565
ISSN:1000-2324
年,卷(期):2024.55(1)
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