Arbuscular mycorrhizal fungi(AMF)can form a symbiotic connection with most terrestrial plants,promote the absorption and utilization of mineral nutrients by roots,and enhance plant productivity and stress resistance.We analyzed the AMF in rhizosphere soils of 10-year-old and 30-year-old Malus robusta by high-throughput sequencing technology.The results showed that the OTUs number of 10-year-old and 30-year-old M.robusta was similar,but there were only 6 common OTUs.The analysis of the Sobs index,ACE index,Shannon index and Simpson index showed that the richness and diversity of AMF in rhizosphere soils of 10-year-old M.robusta were higher than those of 30-year-old M.robusta.In community composition analysis,the number of AMF dominant species in rhizosphere of 10-year-old M.robusta was higher than that of 30-year-old M.robusta,and species abundance was more uniform.Therefore,M.robusta planting time can affect the diversity of AMF,with the increase of planting time,the diversity of root AMF decreased.