Laccase is a copper-containing polyphenol oxidase with a wide range of application,including bio-detection,industrial dye decolorization,organic pesticide degradation,pulp bleaching,and the food and beverage industries.We utilized a high-yield laccase strain of Tramete hirsuta identified in the laboratory.The laccase was separated and purified through ammonium sulfate precipitation and DEAE Sepharose FF anion exchange chromatography.The enzyme activity yield was 57.2%,with a purification fold of 6.0 and a specific activity of 758.5 U/mg.The molecular weight of laccase was about 50 kDa.The crude enzyme solution from Tramete hirsuta was used to decolorize crystal violet,bromophenol blue,malachite green,and Janus green B.The effects of dye concentration,temperature,pH and NaCl on the decolorization rate of bromophenol blue and malachite green were also investigated.The decolorization rates were higher when the dye concentration was 40 mg/L for bromophenol blue and 50 mg/L for malachite green.The decolorization rates of laccase on bromophenol blue and malachite green were 68.51%and 83.06%,respectively,at the temperature of 50℃.Bromophenol blue exhibited the highest decolorization rate of 72.61%at pH 3.5,while malachite green showed the highest decolorization rate of 83.49%at pH 4.5.NaCl had an inhibitory effect on the dye decolorization catalyzed by Trametes hirsuta laccase.Our study showed Trametes hirsuta laccase has a great application potential in dye decolorization and industrial wastewater treatment.
Trametes hirsutalaccaseisolation and purificationdye decolorization
维普
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