Molecular Mechanism of Interaction of Rhizoctonia solani Rhi-miR9829-5p with Rice and Soybean
The fungus Rhizoctonia solani can infect over 260 plant species including rice and soybean,making it one of the major fungal pathogens in crop production.microRNAs(miRNAs)are a class of non-coding RNA molecules composed of 20-25 nucleotides,which can regulate the expression of target genes at the transcriptional or translational level by degrading or inhibiting mRNA.Previous studies have shown that miRNAs are involved in various biological processes such as fungal-plant interactions.In earlier research,a miRNA,Rhi-milR9829-5p,was identified by the research team to negatively regulate maize resistance to diseases.To further study its pathogenic functions and mechanisms in different crops,this study obtained transgenic rice plants overexpressing Rhi-milR9829-5p mature bodies.Upon inoculation with Rhizoctonia solani,it was found that the transgenic rice plants with overexpressed Rhi-milR9829-5p showed significantly reduced resistance to sheath blight.Bioinformatics methods were used to predict downstream target genes of Rhi-milR9829-5p in rice and soybean and to conduct functional annotation analysis.The results indicated that Rhi-milR9829-5p regulates the resistance of rice and soybean to diseases through multiple biological pathways,with distinct mechanisms observed between the two crops.The research results provide preliminary insights into the molecular mechanisms by which Rhi-milR9829-5p from Rhizoctonia solani regulates disease resistance in rice and soybean,further confirming the cross-species regulatory function of miRNAs and offering genetic resources for molecular breeding of disease-resistant crops.
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