FtsZ protein,as a homolog of tubulin,often assembles into a contractile cytoskeletal element known as the"Z"ring,influencing plastid division.However,research on cucumber FtsZ proteins has not been reported,and its function is unclear.In this paper,we identified three FtsZ proteins in cucumber by bioinformatics methods and classified them into two groups.Sequence analysis revealed that their lengths range from 4 493 to 6 264 bp,containing 6 to 7 exons,with amino acid lengths of 421 to 488 AA,located on chromosome 3 or 6;subcellular localization predictions indicated that cucumber FtsZ is located in the chloroplast or cytoplasm;RNA-seq analysis found that cucumber FtsZs are highly expressed in stem,leaf,female flower,and ovary,and fluorescence quantitative PCR results showed that the expression level of cucumber CsFtsZ2-1 gene is also high in male flowers,indicating that cucumber FtsZs genes play a role in the growth and development of leaves,flowers,and ovaries;additionally,in the process of responding to coercion,the CsFtsZ2-1 gene can respond to salt stress,CsFtsZ1,CsFtsZ2-1,and CsFtsZ2-2 can respond to cold stress,and CsFtsZ1 and CsFtsZ2-1 can respond to powdery mildew pathogen infection.The research results provide a reference and basis for further study of the role and mechanism of FtsZs genes in cucumber.
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