Cucumber(Cucumis sativus L.)is an important vegetable crop widely grown worldwide.The Hsp70 subfamily of heat shock protein(Hsp)genes is considered the most important molecular chaperone proteins involved in the heat shock response in plants.However,little is known about the function of Hsp70 genes in cucumber.The construction of cucumber pan-genome provides platform resources and information for fully exploiting genetic variation resources and cucumber genetic breeding.In this study,we identified and analysed the Hsp70 genes in the pan-genome based on 11 different cucumber accessions(including three wild species and eight cultivars)by bioinformatic technology,and identified a total of 12 CsHsp70s genes,of which nine genes were common to all accessions,and five common genes were identical in protein sequence length,gene structure and conserved protein motifs(CsHsp70-1,CsHsp70-2,CsHsp70-7,CsHsp70-8,CsHsp70-11),suggesting that their functions may be conserved in different cucumber accessions;The other four common genes(CsHsp70-3,CsHsp70-4,CsHsp70-5,CsHsp70-12)did not have identical gene structures,protein sequence lengths,and conserved protein motifs in different cucumber accessions,suggesting that their functions in different cucumber accessions might be diverse.Three non-common genes(CsHsp70-6,CsHsp70-9,CsHsp70-10)were also identified.The occurrence of non-common genes was due to their deletion in the Indian cultivar'Hx14'.This gene loss phenomenon may be related to the long process of domestication and breeding of cucumber.This study provides a reference and basis for the functional study of Hsp70 genes and molecular genetic breeding for heat tolerance in cucumber.
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