高温响应基因MdMYB308抑制苹果花青苷合成
MdMYB308:High-Temperature-Inducible Gene Suppressing Anthocyanin Accumulation in Apples
房月 1吴志蒙 1刘春帆 1徐同尧 1李婷 1王海波 2冯守千1
作者信息
- 1. 山东农业大学园艺科学与工程学院,山东 泰安 271018
- 2. 山东省果树研究所,山东 泰安 271000
- 折叠
摘要
高温抑制苹果花青苷合成,从而影响果实的着色.本研究发现高温条件下,MdMYB308基因的表达被强烈诱导.通过亚细胞定位实验,发现MdMYB308定位在细胞核中.为了研究MdMYB308的功能,我们利用过表达和CRISPR/Cas9基因编辑技术对其进行了探究.结果显示,MdMYB308抑制了苹果花青苷结构基因的表达和花青苷合成,MdMYB308在高温抑制苹果花青苷合成中起到了重要的作用,在耐高温红色苹果育种中具有重要的应用潜力.
Abstract
High temperatures suppress anthocyanin synthesis in apples,thereby affecting the fruit's coloration.This study found that the expression of the MdMYB308 gene was strongly induced under high-temperature conditions.Subcellular localization experiments revealed that MdMYB308 is predominantly localized in the cell nucleus.To investigate the function of MdMYB308,we explored it using overexpression and CRISPR/Cas9 gene editing techniques.The results demonstrated that MdMYB308 suppresses the expression of anthocyanin structural genes and anthocyanin synthesis.MdMYB308 plays a significant role in suppressing anthocyanin synthesis under high temperatures and has significant potential for application in breeding heat-tolerant red apples.
关键词
苹果/高温/花青苷/MdMYB308Key words
Apple/high temperature/anthocyanin/MdMYB308引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据