Preparation of Perovskite Quantum Dots-molecularly Imprinted Fluorescent Probe and Application in Enrofloxacin Detection
Enrofloxacin is a quinolone antibiotic,and long-term consumption of food with excessive enrofloxacin can pose health risks to humans.Perovskite quantum dots(PQDs)were synthesized by the hot-injection method,with enrofloxacin as the template molecule,and combined with molecular imprinting technology to prepare a fluorescent probe based on perovskite quantum dots-molecularly imprinted polymer(PQDs@MIP)composite material.The morphology,functional groups,and pore characteristics of the material were investigated using transmission electron microscopy,Fourier transform infrared spectroscopy,and nitrogen adsorption-desorption experiments.The effects of incubation time,enrofloxacin concentration,and analogs on the fluorescence response behavior of both were explored.The results indicated that enrofloxacin could significantly quench the fluorescence of PQDs@MIP at 514 nm,with fast quenching kinetics(15 min)and a high saturation concentration(150 mg L-1).Moreover,there is a strong linear correlation between the quenching degree and concentration within the range of enrofloxacin concentrations from 0.05 to 40.0 mg·L-1.Based on this,a fluorescence sensing detection method for enrofloxacin was constructed,with a detection limit of 0.02 mg·L-1.The recovery rates for the addition of three aquatic products were 93.6%to 106.5%,with relative standard deviations of 1.9%to 4.9%,proving its outstanding potential for practical application.
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