分子印迹电化学发光传感检测预制菜中的恩诺沙星
Molecularly Imprinted Electrochemiluminescence Sensor for Detection of Enrofloxacin in Prefabricated Dishes
于润泽 1高春慧 1徐志祥1
作者信息
- 1. 山东农业大学食品科学与工程学院,山东 泰安 271018
- 折叠
摘要
本文基于石墨相氮化碳纳米片(g-C3N4 NSs)和分子印迹聚合物制备了一种分子印迹电化学发光(MIP-ECL)传感器用于特异性检测恩诺沙星(ENR).采用出色电化学发光性能的g-C3N4 NSs作为发光体,以ENR为模板分子,邻苯二胺为功能单体,在修饰电极表面通过循环伏安法制备分子印迹聚合物膜.在最优条件下,该MIP-ECL传感器对ENR的线性响应范围为5×10-9～1×10-5 mol/L,最低检出限为9.6×10-10 mol/L.此外,传感器在实际样品中加标回收率为98.30%～106.20%.研究表明,该传感器具有成本低、灵敏度高、选择性好等优点,非常适合用于预制菜等食品中恩诺沙星的快速筛查.
Abstract
In this paper,a molecularly imprinted electrochemical luminescence(MIP-ECL)sensor was developed for the specific detection of enrofloxacin(ENR)based on graphite phase carbon nitride nanosheets(g-C3N4 NSs)and molecularly imprinted polymers.The g-C3N4 NSs with excellent electrochemical luminescence properties was used as luminophore.With ENR as the template molecule and o-phenylenediamine as the functional monomer,molecularly imprinted polymer film was prepared on the surface of a modified electrode through cyclic voltammetry.Under optimal conditions,the MIP-ECL sensor exhibited a linear response range for ENR from 5×10-9 to 1×10-5 mol/L,with a detection limit as low as 9.6×10-10 mol/L.Additionally,the sensor demonstrated a spiked recovery rate in actual samples ranging from 98.30%to 106.20%.The research indicates that the sensor possesses advantages such as low cost,high sensitivity,and good selectivity,making it highly suitable for the rapid screening of enrofloxacin in pre-packaged foods and other food products.
关键词
电化学发光/分子印迹聚合物/恩诺沙星/石墨相氮化碳纳米片/预制菜Key words
Electrochemiluminescence/molecularly imprinted polymer/enrofloxacin/g-C3N4 nanosheets/prefabricated dishes引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据