Analysis of Molecular Characterization of EuNPC1-Like Gene in Eucommia ulmoides
Sterol transporters are known to have a significant impact on various aspects of plant physiology,including growth and development,stress tolerance,and the regulation of plasma low density lipoprotein and cholesterol levels in the human body.Eucommia ulmoides,a Chinese medicinal material renowned for its sterol content,has various health benefits such as regulating blood pressure.However,little is known about the molecular characteristics of the sterol transporter NPC1 and its corresponding gene in E.ulmoides.In this study,the EuNPC1-Like gene of Eucommia ulmoides was identified,analyzed,and characterized using knowledge from the human NPC1 gene.The results showed that the EuNPC1-Like protein had a total length of 3876 base pairs and was characterized as an unstable hydrophobic lipid soluble protein.This protein possessed 20 glycosylation sites and 136 phosphorylation sites.It was a transmembrane protein consisting of 13 α helices,primarily localized in lysosomes/vacuoles and cell membranes.Furthermore,it contained a conserved cholesterol transport domain known as NPC 1_N,which exhibited a three-dimensional structure similar to that of NPC1 protein in humans and Arabidopsis.Additionally,the protein contains cis-acting elements that responded to various stimuli such as light,gibberellin,low temperature,drought,anaerobic conditions,and methyl jasmonate.The NPC1 protein was closely associated with high oil content plants such as sunflower,olive,coffee,and pinus camphoris.Network pharmacological analysis suggested that the NPC 1 protein played a role in regulating phytosterol metabolism.Additionally,Eucommia β-sitosterol targeted human fat and sterol metabolism,which was similar to sterol metabolism in plants and animals.This study provides a basis for further understanding the function of the EuNPC1-Like gene.
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