Occurrence and Control Measures of Browning in Plant Tissue Culture
Browning is the most prominent problem affecting plant tissue culture,which seriously affects the growth and differentiation of culture,and even causes plant death.The research results at home and abroad showed that the mechanism of browning was mainly oxidative browning caused by phenols accumulation and oxidation,and the main influencing factors were plant varieties and genotypes,physiological conditions of explants,culture environment,disinfection methods and other tissue culture methods.The prevention and control measures based on the occurrence mechanism and influencing factors of browning were to select explant materials,which were not easy to browning,optimize the medium compositions and culture conditions,and add additives to inhibit browning.At present,the research on the mechanism of browning could not fully explain the oxidative browning,and the control measures may have adverse effects on other indexes of tissue culture.
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NSTL
万方数据
维普
