首页|孔雀石绿对稀有鮈鲫的胚胎发育毒性研究

孔雀石绿对稀有鮈鲫的胚胎发育毒性研究

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本研究以稀有鮈鲫(Gobiocypris rarus)为实验对象,探究不同浓度孔雀石绿对稀有鮈鲫胚胎的致畸性和毒性效应.在稀有鮈鲫通过干法人工授精发育 4.5 hpf,通过设置1 个空白对照组及5 个浓度组(1.0、1.5、2.0、2.5 和3.0 mg/L),采用水体浸泡暴露的实验方式研究孔雀石绿对胚胎的影响.结果表明孔雀石绿会导致稀有鮈鲫自主运动频次降低、心跳次数降低、孵化率也大大降低.高浓度(2.5、3.0 mg/L)的孔雀石绿对于稀有鮈鲫胚胎孵化有较高致死效应,低浓度(1.0~2.0 mg/L)的孔雀石绿对于稀有鮈鲫胚胎孵化有显著影响,影响稀有鮈鲫的自主运动、心跳和孵化能力,并且稀有鮈鲫在孔雀石绿的暴露下导致畸形症状,如尾部弯曲、心包囊水肿等.
Embryonic Developmental Toxicity of Malachite Green to Gobiocypris rarus
In this paper,Gobiocypris rarus was used as the experimental object to explore the teratogenic and toxic effects of malachite green at different concentrations on embryos of G.rarus.Whose was developed for 4.5 hpf by dry artificial insemination,and the effects of malachite green on embryos was studied by water immersion exposure by setting a blank control group and five concentration groups(1.0 mg/L,1.5 mg/L,2.0 mg/L,2.5 mg/L and 3.0 mg/L).The results showed that the frequency of voluntary movements,the number of jumps,and the hatchability of G.rarus would be decreasing after the malachite green entered.The malachite green in high concentration of 2.5 mg/L and 3.0 mg/L has a high lethal effect on the hatching of G.rarus embryos,while that in low concentration of 1.0 mg/L and 2.0 mg/L has a significant effect on the hatching of G.rarus embryos,affecting the voluntary movement,heartbeat,and hatching ability of G.rarus.G.rarus exposed to malachite green was results in malformation symptoms,such as tail curvature,pericardial cyst edema and so on.

malachite greenGobiocypris rarusembryotoxic effects

杨婷、曾鹏、冉盛菊、马秀慧、黄福江

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贵州大学动物科学学院,贵州贵阳 550025

贵州省水产研究所,贵州贵阳 550025

孔雀石绿 稀有鮈鲫 胚胎毒性效应

贵州省科技计划贵州省科技计划

黔科合支撑[2023]一般107黔科合支撑[2020]1Y099

2024

山地农业生物学报
贵州大学

山地农业生物学报

CSTPCD
影响因子:0.486
ISSN:1008-0457
年,卷(期):2024.43(2)
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