摘要
为探究浅色戈卢别夫氏菌培养液对烟草种子萌发及幼苗生长的影响,以烤烟品种'K326'为试验材料,用不同浓度(原液、1/10 原液、1/50 原液、1/100 原液、1/200 原液)浅色戈卢别夫氏菌(Golubevia pallescens)培养液对烟草种子进行浸种处理,分析其对种子发芽势、发芽率、发芽指数以及根长的影响;用3 个浓度(原液、1/50 原液、1/100 原液)浅色戈卢别夫氏菌培养液喷施烟草幼苗叶片,分析其对烟草幼苗生长及抗氧化代谢酶活性的影响.结果表明:1/100 原液浸种处理对烟草种子萌发具有促进作用,发芽势、发芽指数和根长显著高于对照;喷施烟草幼苗叶片后,1/50 原液可显著提高叶长、叶宽、株高、叶绿素a、叶绿素b、类胡萝卜素含量和过氧化氢酶(CAT)活性;1/100 原液可显著提高烟草幼苗的根系活力,显著降低多酚氧化酶(PPO)活性.综上,浅色戈卢别夫氏菌培养液能显著促进烟草种子萌发和根的伸长,并显著促进烟草幼苗生长,提高其抗氧化能力.
Abstract
To explore the effect of light colored Golubevia pallescens culture solution on seed germination and seedling growth of tobacco.The flue-cured tobacco variety'K326'was as the experimental material,to evaluate their effects of light-colored G.pallescens culture solution on seed germination potential,germination rate,germination index,and root length of tobacco,which soaked in different concentrations(stock solution,1/10 stock solution,1/50 stock solution,1/100 stock solution,1/200 stock solution).Meanwhile,effects of tobacco seedling growth and antioxidant enzyme activities were analyzed after tobacco seedling leaves were sprayed with three concentrations(stock solution,1/50 stock solution,and 1/100 stock solution)of light colored G.pallescens culture solution.The results showed that the germination of tobacco seeds soaked with 1/100 of the original solution was promoted,and which germination potential,germination index and root length were significantly higher than those of the control.After spraying the leaves of tobacco seedlings with 1/50 of the original solution,of which the leaf length,leaf width,and plant height were significantly higher than those of the control.The content of chlorophyll a,chlorophyll b,and carotenoids was significantly higher than that of the control,significantly reducing the activity of polyphenol oxidoreductase(PPO),and increasing the activity of catalase(CAT),peroxidase(POD)activity.G.pallescens culture solution with 1/100 stock solution significantly increased the root activity of tobacco seedlings.In summary,light-colored G.pallescens culture medium could significantly promote tobacco seed germination and root elongation,and significantly promote the growth of tobacco seedlings and improve its antioxidant capacity.
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