Cloning,Bioinformatics Analysis and Temporal Expression of the Fatty Acid Synthase Gene in Aspongopus chinensis Dallas
Aspongopus chinensis Dallas,1851,is a traditional medicinal and edible insect in China,with an increasing demand.Nevertheless,it shows strict reproductive diapause,which is influenced by Fatty Acid Synthase(FASN).In this study,specific primers were designed for FASN based on the transcriptome data of A.chinensis,and total RNA was extracted and cloned using PCR.The FASN gene,known as AcFASN,was successfully cloned through the process of RNA extraction,reverse transcription,PCR,and ligation transformation.Bioinformatics and homologous evolutionary tree analysis were conducted using the gene sequence of FASN,while the temporal expression graphs of AcFASN were generated through real-time fluorescence quantitative PCR.The purpose of these analyses was to determine if AcFASN plays a role in diapause regulation.The results revealed that the AcFASN gene spans a total length of 7092 bp and codes for 2363 amino acids.Bioinformatics analysis showed that AcFASN was a hydrophilic protein that was non-secreted and was found in the cytoplasm without a transmembrane region or signal peptide.Phylogenetic tree analysis revealed a strong evolutionary connection between the FASN amino acid sequences of A.chinensis and Halyomorpha halys.Temporal expression analysis showed that the AcFASN expression level was elevated during diapause,suggesting that AcFASN may play a role in regulating the growth and development of A.chinensis by modulating its expression in the body.
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