Genome-wide Identification and Expression Analysis of the GEF Gene Family under Drought Stress in Six Gramineae Plants
The small G protein-mediated guanine trinucleotide phosphate/guanine dinucleotide phosphate(GTP/GDP)signaling pathway plays a critical role in plant resistance,with the guanine nucleotide exchange factor(GEF)controlling various diminutive G proteins.Studies have shown that plant genomes contained at least two types of GEF genes,BIG(Brefeldin A-inhibited guanine nucleotide exchange protein)and GBF(Golgi brefeldin A-resistant guanine nucleotide exchange factor).However,the systematic identification and expression analysis of these GEF genes remained unresolved.Consequently,this study conducted a comparative examination of GEF gene clusters in the grass family through gene family identification.The findings demonstrated that 58 GEF genes were obtained from the genomes of six Gramineae species(Oryza sativa,Triticum aestivum,Sorghum bicolor,Aegilops tauschii,Setaria italica,and Zea mays),and these genes were located on 45 different chromosomes.Phylogenetic analysis indicated that the Gramineae GEF gene family was categorized into five subfamilies(BIG1/4,BIG2/3,BIG5,GNL2,GNON)with distinct single copy and selective retention patterns.The covariance analysis revealed a intricate complex evolutionary history of the GEF genes within the grass family,while the results of drought-induced expression further confirmed the divergence of the GEF gene family in terms of drought resistance across different gramineae,subfamilies,and species.These findings will be valuable in identifying drought resistance genes in graminaceous crops.
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万方数据
