Optimization of Medium Containing Yellow Syrup Water and Effects of Growth-promoting Bacteria Culture on Seed Germination
Yellow syrup water contains a large number of nutrients,which is of great significance to the reuse of its resources.The screening of growth-promoting strains with ammoniation and phosphorus-solubility was carried out by qualitative analysis of hydrolysis circle and quantitative determination of NH4+-N content.The fermentation medium containing yellow syrup water of the two strains was optimized by single factor test and orthogonal test.Ryegrass and alfalfa seeds were soaked in the fermentation culture of the two strains to analyze their effects on seed germination.The results showed that Pseudomonas putida HGD3 and Burkholderia pyrrocinia P10 had the strong ammoniation ability and phosphorus solubility.The NH4+-N concent of HGD3 and P10 strains cultured for 48 h in the optimized medium containing 40%yellow syrup water was 894.92 mg/L and 546.49 mg/L,respectively,and which was 6.79 and 2.60 times higher than that before optimization.The soaking of HGD3 and P10 strains significantly increased the germination rate of ryegrass and alfalfa seeds,which were 22.66%and 16%higher than the control,respectively.After the treatments of HGD3 and P10 strains,the bud length of ryegrass increased by 2.57 times and 1.77 times,and root length increased by 2.53 times and 1.90 times,respectively.It showed that the culture of Pseudomonas putida HGD3 in medium containing yellow syrup water could significantly promote the germination of ryegrass and alfalfa seeds,especially ryegrass.The results provide a theoretical basis for the reuse of yellow syrup water and the promotion of the two strains on the seed germination.
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