Establishment of real-time PCR method for rapid qualitative detection of streptococcus faecalis in PC barrel
A real-time fluorescent PCR method was developed for rapid qualitative detection of Streptococcus faecalis in PC drinking water barrels.The 23S rDNA gene sequence of Streptococcus faecalis was amplified and detected by using specific primers and probes.The specificity and repeatability of our method was evaluated based on the comparative use of our method and the national standard method to detect PC drinking water bucket samples.The results indicate that our method can quickly and qualitatively detect Streptococcus faecalis in PC drinking water barrels,effectively shorten detection time and reduce experimental operations,with high specificity and repeatability.
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