PC饮用水桶中粪链球菌快速定性检测实时荧光PCR方法的建立
Establishment of real-time PCR method for rapid qualitative detection of streptococcus faecalis in PC barrel
赵彤彤 1李娜 1王一村 1郗丹 1张宇鑫 1高静静 1周莉莉 1王智 1温展1
作者信息
- 1. 山东省产品质量检验研究院,山东 济南 250102
- 折叠
摘要
开发了一种针对PC饮用水桶中粪链球菌快速定性检测的实时荧光PCR方法,该方法基于粪链球菌23S rDNA基因序列,使用特异性引物和探针进行扩增和检测.对其特异性和重复性进行评估,并分别应用该方法和国标方法对PC饮用水桶样品进行了检测.结果表明,该方法能够对PC饮用水桶中粪链球菌进行快速定性检测,可有效缩短检测时间并减少实验操作,具有较高的特异性和重复性.
Abstract
A real-time fluorescent PCR method was developed for rapid qualitative detection of Streptococcus faecalis in PC drinking water barrels.The 23S rDNA gene sequence of Streptococcus faecalis was amplified and detected by using specific primers and probes.The specificity and repeatability of our method was evaluated based on the comparative use of our method and the national standard method to detect PC drinking water bucket samples.The results indicate that our method can quickly and qualitatively detect Streptococcus faecalis in PC drinking water barrels,effectively shorten detection time and reduce experimental operations,with high specificity and repeatability.
关键词
PC饮用水桶/桶装饮用水/粪链球菌/快速定性检测/实时荧光PCRKey words
polycarbonate(PC)barrel/barreled drinking water/Streptococcus faecalis/rapid qualitative testing/real-time PCR method引用本文复制引用
基金项目
2021年度山东省市场监管局科技计划项目(2021MR16)
出版年
2024
NETL
NSTL
万方数据