ARTP method to improve the fermentation laccase of Trametes versicolor ATCC20869
Trametes versicolor laccase is a copper-containing polyphenol oxidase that can oxidize a variety of phenolic and aromatic compounds.Its main product is water,and it produces almost no toxic by-products.While it has good application prospects,the low yield of Trametes versicolor laccase limits its further application.This study used Atmospheric and Room Temperature Plasma(ARTP)technology to successfully mutate a new strain,TA-03,from Trametes versicolor ATCC20869.To enhance laccase production,the paper optimized fermentation conditions through single-factor shake flask experiments and response surface analysis.The results showed that under optimized conditions,with a farnesol addition of 4.48 mmol/L,copper sulfate addition of 1.90 mmol/L,and pH maintained at 4.00,the laccase production of the mutant strain reached(283.77±8.91)U/mL.To explore the reasons for the increased laccase activity of the mutant strain TA-03,resequencing and transcriptome analysis were performed.The research results show that the mutation of amino acids in the active region of laccase and the enrichment of metabolism and other aspects lead to the improvement of laccase activity.This provides a meaningful exploration of the practical application of Trametes versicolor laccase and enhances the application of laccase in the fermentation industry.
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