Extracellular vesicles carryabundant biological molecules,including nucleic acids,proteins and lipids,which play im-portant roles in intercellular communication and are closely related to the occurrence and development of many diseases.Extracellular vesicles are widely distributed in most body fluids and are one of the effective biomarkers for liquid biopsy,which can be used for non-invasive early diagnosis of disease and efficacy monitoring.Therefore,the analysis and study of extracellular vesicles and their carrying biomolecules(proteins,nucleic acids)have received extensive attention.Based on the specific recognition of extracellular vesicles sur-face proteins by antibodies or nucleic acid aptamers,various strategies have been proposed for the highly sensitive and highly specific detection of extracellular and its surface proteins.In situ and accurate detection of extracellular nucleic acid can be achieved by direct delivery or membrane fusion of the sensing probe into extracellular.This review focuses on the recent advances of detection methods for extracellular vesicles and their carrying biomolecules and theirfuture developments are proposed.
国家科技期刊平台
NSTL
万方数据
