In vitro and in vivo studies on bone defect repair in cleft lip and palate with BMP-2/mBGPs/PLGA composite scaffolds
For the preparation of the ability to promote osteogenesis and angiogenesis artificial porous biomaterial scaffolds and ex-plore its performance,we sythesized large-diameter mesoporous bioactive glasses(mBGPs)through hydrothermal synthesis and the in-corporation of pore-expanding agents.Bone morphogenetic protein-2(BMP-2)was loaded into the mesopores of mBGPs using vacu-um impregnation method.Subsequently,BMP-2/mBGPs and PLGA composite scaffolds were prepared by solvent casting particle leac-hing method.The material characterization results showed that mBGPs were hollow spherical shells with an amorphous phase structure and a disordered three-dimensional foam pore structure,which could continuously dissolve calcium ions,phosphate ions and silicate i-ons.Meanwhile,mBGPs had the ability of protein loading and slow release.The in vitro experimental results indicated that the BMP- 2/mBGPs/PLGA porous scaffold enhanced the adhesion and proliferation of rat bone marrow mesenchymal stem cells(rBMSCs)and significantly increased the secretion and expression of bioactive substances.In the rat alveolar bone defect model,the scaffold acceler-ates the growth rate and maturation of new bone tissue.
Treatment of cleft lip and palateBioactive materialsMesoporous materialsLoading growth factorBone tissue engi-neering scaffoldRepair of bone defects
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