细胞悬液浓度对常规培养微核法微核检测结果的影响
The effect of cell suspension concentration on the results of conventional culture micronucleus detection
邝珠芳 1谢颖 1郭庆林 1沈天禹 1杨淑欢 1吴衍恒1
作者信息
- 1. 广东省中山市疾病预防控制中心,广东 中山 528400
- 折叠
摘要
目的 探讨出更快捷、统一、有效的方法提高微核的检出率和阅片效率,为职业放射卫生人员、有其他职业暴露人群的健康检查提供实验依据.方法 人外周血淋巴细胞微核试验培养法在滴片时设定四个不同浓度的细胞悬液(细胞和上清液体积比分别为1∶1、1∶2、1∶3、1∶4),制得微核标本,显微镜下对比分析制片后的染色效果及形态分布,记录阅片时间,算出淋巴细胞微核率及微核细胞率.结果 显微镜下阅片分析认为1∶2和1∶3浓度微核片染色效果及形态分布较为理想.不同浓度下的阅片时间有显著差异(F=576.12,P＜0.05),阅片时间均值大小排序为1∶4浓度＞1∶3浓度＞1∶2浓度＞1∶1浓度.不同浓度下的微核细胞数没有显著差异.1∶1浓度微核片的微核细胞检出率最低.结论 通过对比分析得出1∶(2-3)的细胞悬液浓度制片效果较为理想,能提高阅片效率.
Abstract
Objective To explore a faster,unified,and effective method to improve the detection rate and film reading efficiency of micronuclei,and to provide experimental basis for the health examination of occupational radiation health personnel and other occupational exposed populations.Methods Human peripheral blood lymphocyte micronucleus test culture method:four different concentrations of cell suspensions(cell and supernatant volume ratios were 1∶1,1∶2,1∶3,1∶4,respectively)were set at the time of dropping,and micronucleus samples were prepared.The staining effect and morphological distribution after preparation were compared and analyzed under the microscope,and the film reading time was recorded to calculate the lymphocyte micronucleus rate and micronucleus cell rate.Results The results of microscopic examination showed that the staining effect and morphological distribution of micronucleus slides at concentrations of 1∶2 and 1∶3 were relatively ideal.There was a significant difference in the reading time under different concentrations(F=576.12,P＜0.05),and the average reading time was ranked as 1∶4 concentration＞1∶3 concentration＞1∶2 concentration＞1∶1 concentration.There was no significant difference in the number of micronucleus cells under different concentrations.The detection rate of micronucleus cells in micronucleus slides with a 1∶1 concentration was the lowest.Conclusion Producing high-quality micronucleus specimens can improve the detection rate and film reading efficiency of micronuclei.Through comparative analysis,it can be concluded that the production effect of cell suspension concentration of 1∶(2-3)is relatively ideal,which can improve the efficiency of film reading and the detection rate of micronuclei.
关键词
淋巴细胞/微核试验/微核细胞率/阅片效率/检出率Key words
Lymphocytes/Micronucleus test/Micronucleus cell rate/Film reading efficiency/Detection rate引用本文复制引用
出版年
2025
NETL
NSTL
万方数据