Hypoglycemic effect of different extracts of Cistanche deserticola on db/db mice
Objective To explore the hypoglycemic effect and mechanism of different extracts of Cistanche deserticola and processed C.deserticola on db/db mice.Methods The total polysaccharides,total oligosaccharides and total glycosides of C.deserticola and processed C.deserticola were enriched,and their contents were compared and analyzed.Select db/db mice as experimental animals.After 28 days of administration,record the body weight,food intake,water consumption,blood glucose,serum insulin(INS),glycosylated hemoglobin(HbA1c),liver triglyceride(TG),total cholesterol(TC),low density lipoprotein(LDL-C),high density lipoprotein(HDL-C),malondialdehyde(MDA),superoxide dismutase(SOD),uric acid(UA)in plasma and urine creatinine(Cr)and urinary microalbumin(mALB)levels;Pathological sections of pancreas and kidney were prepared by HE staining;The expression level of type-Ⅳ collagen protein in kidney was determined by immu-nohistochemistry.Results The content of polysaccharide in C.deserticola decreased after wine steaming,the content of beta-ine in the polysaccharide in C.deserticola increased after wine steaming,the content of total glycosides decreased slightly af-ter wine steaming,the content of verbascoside decreased,and the content of isoacteoside increased.Compared with the model group,the fasting blood glucose(FBG),oral glucose tolerance(OGTT),HbA1c,insulin resistance index(HOMA-IR),HO-MA-β,MDA,LDL-C,UA,Cr and mALB in the different extracts of C.deserticola and processed C.deserticola were signifi-cantly decreased,INS and HDL-C showed an upward trend(P＜0.05,P＜0.01),and can improve pathological damage to the pancreas and kidneys,as well as the expression of type-Ⅳ collagen.Conclusion The total glycosides of C.deserticola and processed C.deserticola have better hypoglycemic effects,while polysaccharides and oligosaccharides of processed C.deserti-cola also have certain hypoglycemic effects.
万方数据
维普
