Effect of Human Immunoglobulin(pH4)for Intravenous Injection on complement system in vitro
Objective To evaluate whether Human Immunoglobulin for Intravenous Injection can activate the comple-ment system by complement incubation experiments in vitro and to study the correlation between the complement system and anaphylactoid reactions during the administration of this product.Methods Products from four enterprises were selected,and also were heated to obtain samples with elevated levels of polymers.After incubating the samples with human serum for 1 hour in vitro,the contents of the representative intermediate products of the complement system activation,and the terminal complex Sc5b-9 were determined by ELISA kits.Results Compared with the normal saline control,the complement mole-cules C3a,C4a,C5a,Bb,and Sc5b-9 in the experimental group basically showed a downward trend;After heating the prod-ucts,there was no significant increase in the complement molecules described above.Conclusion Human Immunoglobulin for Intravenous Injection cannot cause anaphylactoid reactions by activating the complement system.On the contrary,it inhib-ited the complement system to a certain extent.
Human Immunoglobulin for Intravenous InjectionPolymerComplement systemInhibitionAnaphylactoid reactions
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