Study of the protective effect of Lactic Acid Bacteria Complex Enteric Capsules strengthen Shenshuaining Capsules on renal fibrosis
Objective To investigate the protective effect of Lactic Acid Bacteria Complex Enteric Capsules(LCC)combined with Shenshuaining Capsules(SSN)on renal fibrosis in the ureteral obstruction(UUO)surgical rat models.Meth-ods LCC were tested.The UUO surgery was performed to establish renal fibrosis model in rat models.After drug combina-tion of LCC and SSN for 21 days,terminal body weight was recorded and the renal coefficient was calculated;BUN,Scr and MAU were detected by biochemical instrument;Kidney tissue morphology and the degree of fibrosis were observed and eval-uated by HE and Masson staining;The expression of α-SMA and Col1 A in kidney tissues was determined by immunohisto-chemistry;The content of IL-6,TNF-α,CRP and TGF-β in renal tissue and the content of D-lactate,endotoxin and diam-ine oxidase(DAO)in serum were detected by ELISA;The mRNA expression of ZO-1,Occludin,Claudin-1,and Claudin-2 in the ileal tissue was determined by Q-PCR.Results Compared to SSN group,the rat models with drug combination of LCC combined with SSN treatment significantly reduced renal coefficient,BUN,Scr and MAU;Increased the body weight;Significantly improved renal tissue morphology and collagen accumulation;Decreased the expression of α-SMA and Col 1 A protein,IL-6,TNF-α,CRP,TGF-p,D-lactate and diamine oxidase(DAO);Increased mRNA expression of ZO-1,Occlu-din,Claudin-1 and decreased mRNA expression of Claudin-2,and ultimately improved renal fibrosis.Conclusion LCC can enhance the improvement of SSN on renal fibrosis,and its effect is mainly related to anti-inflammation,anti-fibrosis,regulation of microflora balance and improvement of intestinal mucosal barrier.Based on the renal-intestinal axis theory,it provides a basis and a new treatment method for the treatment of renal fibrosis.
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