Preparation and evaluation of transdermal drug delivery properties of alendronate sodium liposomes
Objective To screen the optimal conditions for the preparation of alendronate sodium(ALN)liposomes and investigate their in vitro transdermal properties.Methods ALN liposomes were prepared by film hydration combined with periplasmic extrusion,the encapsulation rate and drug loading capacity were calculated,and the permeability of the drug was investigated by using a two-compartment vertical transdermal diffusion cell to calculate the cumulative permeation and the fitting of the release model.Results The optimal conditions for the preparation of liposomes were 2.5∶1 membrane ratio,700 r·min-1 rotational speed,10∶1 lipid-drug ratio,6 min ultrasonication time,45 ℃ hydration temperature,49.6％encapsulation rate and 14％loading efficiency,respectively,and the cumulative permeation amount of ALN liposomes was 814.78 μg·cm-2,which was twice as much as that of the stock solution.The release model of the stock solution was basically in accordance with the Ritger-Peppas equation,and the release model of ALN liposomes was basically in accordance with the zero-order kinetic equation,which has a certain effect of slow and controlled release.Conclusion The prepared ALN liposomes were homogeneous and stable,and the ALN-containing liposomes had a good transdermal effect,thus proving the feasibility of making ALN into liposomes,and also providing ideas for the research of new dosage forms for topical applica-tion,which has a good prospect for development.
万方数据
维普
