Investigation of the mechanism of action of Shutiao Qiji Decoction in the treatment of hypothyroidism based on metabolonics
Objective To investigate the mechanism of therapeutic effect of Shutiao Qiji Decoction on hypothyroidism by analyzing serum markers in hypothyroidism rats using non-targeted metabolomics method.Methods The rats were ran-domly grouped into modeling and normal groups after one week of adaptive feeding,and the modeling rats were administered propylthiouracil by gavage for 10 days to model,while the normal group was given the same volume of drinking water by ga-vage.At the end of the modeling period,the rats were tested to determine whether the serum indexes met the diagnostic crite-ria of clinical diseases and the status of the rats during the modeling period to determine the success of the modeling.The rats were grouped into model group,positive drug(eugenol)group,Shutiao Qiji Decoction low concentration group,Shutiao Qiji Decoction medium concentration group and Shutiao Qiji Decoction high concentration group,and administered with the same volume of drinking water for 6 weeks in the normal group and model group.6 weeks later,blood was collected from the abdominal aorta of the rats,and the supernatant was centrifuged for ELISA kit detection and non-targeted metabolomics de-tection.Results Shutiao Qiji Decoction could significantly reduce TSH levels and significantly increase FT3 and FT4 ex-pression levels in hypothyroid rats(P＜0.05).When comparing the normal group with the model group,there were 312 sig-nificantly different metabolites,of which 111 metabolites were up-regulated and 198 metabolites were down-regulated;When comparing the high dose group with the model group,there were 91 significantly different metabolites,of which 47 me-tabolites were up-regulated and 39 metabolites were down-regulated,mostly alkaloids,condensates and polyketides.The metabolic pathways analyzed by KEGG were mainly related to carbon cycle,amino acid biosynthesis and bile secretion.Con-clusion The therapeutic effect of Shutiao Qiji Decoction on hypothyroidism rats is good,which provides an experimental basis for the clinical use and treatment of hypothyroidism with Shutiao Qiji Decoction and a new idea for the Chinese medi-cine of hypothyroidism.
Shutiao Qiji DecoctionHypothyroidismMetabolomicsExperience of famous veteran Chinese medicine practitioners
万方数据
维普
