目的 观察麝香心痛宁抗大鼠血管损伤后内膜增生的作用及可能的作用机制。方法 30只SD大鼠随机分为假手术组、模型对照组和麝香心痛宁组。采用球囊损伤法制备大鼠腹主动脉血管损伤模型，造模后灌胃给予大鼠麝香心痛宁片(200 mg·kg-1)，14 d后将大鼠处死，取其血清及腹主动脉，主动脉血管进行HE染色，检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)、单核细胞趋化因子-1(MCP-1)及谷胱甘肽过氧化物酶(GSH-Px)含量，ELISA法检测大鼠血管组织一氧化氮(NO)、内皮素-1(ET-1)、血管内皮生长因子(VEGF)和白细胞介素-1β(IL-1β)的含量，实时荧光定量PCR和 Western blotting检测Rho、Rock1和Rock2 mRNA和蛋白的表达。结果 与假手术组比较，模型对照组大鼠腹主动脉血管组织内膜增生，腹主动脉血管部位弹力变弱且管壁增厚。麝香心痛宁治疗后血管内膜损伤程度减轻。与模型对照组相比，麝香心痛宁组大鼠血清SOD、GSH-Px活性升高，MCP-1、MDA活性降低(P＜0。05);血管组织NO含量升高，ET-1、VEGF和IL-1β含量降低(P＜0。05);Rho/Rock通路相关因子Rock1、Rock2、Rho的表达降低(P＜0。05)。结论 麝香心痛宁可抑制血管损伤后内膜增生，其作用机制可能与抑制Rho/Rock信号通路有关。
Study on the mechanism of Shexiang Xintongning against neointimal hyperplasia after vascular injury in rats based on Rho/Rock pathway
Objective To observe the effect of Shexiang Xintongning(SXN)on intimal hyperplasia after vascular injury in rats and explore possible mechanisms of action.Methods 30 Sprague-Dawley rats were randomly divided into control,model,and SXN groups.The rat model of abdominal aortic vascular injury was prepared by balloon injury method.Af-ter the model was constructed,SXN(200 mg·kg-1)was given by intragastric administration.After administrated with drugs for 14 days,and the serum and abdominal aortic vascular tissues were collected,and the aortic vessels were stained with HE,the contents of SOD,MDA,MCP-1 and GSH-Px in serum were detected,the contents of NO,ET-1,VEGF and IL-1 β in rat vascular tissues were detected by ELISA,the mRNA and protein expressions of Rho,Rock1 and Rock2 were detected by real-time qPCR and Western blotting.Results Compared with control group,the intima hyperplasia of abdominal aorta in model group was increased,the elasticity of some parts of abdominal aorta was weakened and the tube wall was thickened.The injury degree of vascular intima was reduced after SXN treatment.Compared with model group,the activities of SOD and GSH-Px in serum of SXN group were increased,while the activities of MCP-1 and MDA were decreased(P＜0.05).The contents of NO in vascular tissue were increased,and the contents of ET-1,VEGF and IL-1β were decreased(P＜0.05).The expressions of Rho/Rock pathway-related factors Rock1,Rock2 and Rho were decreased(P＜0.05).Conclusion SXN reduces intimal injury in rats with vascular injury,which may be related to its inhibition of Rho/Rock signaling pathway.
万方数据
维普
