Homogenization extraction process and analysis on antioxidant activities of fucoidan from Sargassum horneri
Objective To optimize the homogenization extraction process of Sargassum horneri fucoidan and determine its antioxidant activity.Methods Based on the extraction yield of fucoidan and single factor experiments,response surface methodology was used to optimize the extraction process of fucoidan from Sargassum horneri,and its antioxidant activity was verified.Results The optimal extraction process conditions were extraction time of 6 min,extraction temperature of 94 ℃,liquid-solid ratio of 34 mg·mL-1,and extraction times of 2 times.Under these conditions,the yield of fucoidan was 14.76％.The results of antioxidant activity research indicate that Sargassum horneri fucoidan has certain antioxidant activity in the mass concentration range of 0.2～10.0 mg·mL-1,with IC50 of 0.22 mg·mL-1 and 0.41 mg·mL-1 for DPPH and ABTS free radical scavenging rates,respectively.Conclusion The extraction process was efficient and can provide a theoretical basis for the preparation and application value of Sargassum horneri fucoidan.
万方数据
维普
