药学研究2024,Vol.43Issue(3) :243-248,287.DOI:10.13506/j.cnki.jpr.2024.03.007

UPLC-Q-Orbitrap-MS快速分析水红花子的化学成分

Rapid identification of the chemical constituents in the fructus of Polygounum orientale by UPLC-Q-Orbitrap-MS

边星 夏林波 邓仕任 孙璐 杨浩
药学研究2024,Vol.43Issue(3) :243-248,287.DOI:10.13506/j.cnki.jpr.2024.03.007

UPLC-Q-Orbitrap-MS快速分析水红花子的化学成分

Rapid identification of the chemical constituents in the fructus of Polygounum orientale by UPLC-Q-Orbitrap-MS

边星 1夏林波 1邓仕任 1孙璐 1杨浩1
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作者信息

  • 1. 辽宁中医药大学药学院,辽宁 大连 116600
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摘要

目的 建立水红花子化学成分的快速鉴定方法.方法 利用超高效液相串联四极杆静电场轨道阱质谱(UPLC-Q-Orbitrap-MS)技术,正、负离子模式下分别采集一级、二级质谱信息.通过推导质谱裂解规律,与质谱数据库(MassBank、mzCloud)、文献数据、对照品比对等方式,对化合物进行指认.结果 从水红花子中共鉴定出54种化学成分,主要包括黄酮类、酚酸类、生物碱类等,其中有15种成分为首次从水红花子中发现,也是首次从药用植物红蓼中发现.结论 该研究可为水红花子的深入质量评价及药效物质基础研究提供依据.

Abstract

Objective To establish a rapid identification method for the chemical components in the fructus of Poly-gounum orientale.Methods The MS1 and MS2 spectral data collected in both positive and negative ion modes was acquired with UPLC-Q-Orbitrap-MS.The constituents were identified by deducing the fragmentation patterns and comparing with mass databases(MassBank,mzCloud),literature data,and reference materials.Results A total of 54 chem-ical components were identified from the fructus of P.orientale,mainly including flavonoids,phenolic acids,alkaloids,etc.A-mong them,15 components were first discovered from the fructus of P.orientale and also from the whole plant of P.orientale.Conclusion This study can provide a basis for in-depth quality evaluation and pharmacological substance basis research for the fructus of P.orientale.

关键词

水红花子/化学成分/超高效液相串联四极杆静电场轨道阱质谱/质谱裂解规律

Key words

Fructus of P.orientale/Chemical constituents/UPLC-Q-Orbitrap-MS/Mass fragmentation patterns

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基金项目

国家自然科学基金(81202857)

辽宁省自然科学基金(20170540617)

出版年

2024
药学研究
山东省药品检验所 山东省药学会

药学研究

CSTPCD
影响因子:0.653
ISSN:2095-5375
参考文献量15
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