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中药山药及其伪品主要化学成分比较分析

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目的 建立中药山药总多糖、总皂苷、总黄酮和总多酚4类活性成分的含量测定方法,比较中药山药及其伪品中成分含量的差异并实现山药正伪品的分类。方法 收集不同产地和来源的28批中药山药和15批伪品,采用紫外-可见分光光度计测定4类成分的含量,并进行多元统计分析。结果 山药及其伪品均含有4类活性成分,且总多糖含量最高,占比约为山药重量的20%,在所测定的4类成分中,占比超90%。基于4类成分的含量可实现山药及其伪品各组之间的分类,并且总多酚的含量对分类的贡献最大。山药各主产区之间成分含量相近,无显著品质差异。结论 实验方法准确、可靠,中药山药4类活性成分的含量可实现山药及其伪品各组之间的分类,为基于功效物质基础的山药质量控制以及山药种植资源提供科学依据。
Comparative analysis on main chemical constituents of Dioscorea opposita and adulterants
Objective To establish a method for the content determination of total polysaccharides,total saponins,total flavonoids and total polyphenols in Chinese yam,and to compare the content difference of components with its adulterants to achieve the classification of Dioscorea opposita and adulterants.Methods The 4 kinds of active components in 28 batches of Chinese yam and 15 batches of adulterants from different origins were determined by UV-visible spectrophotometer,and multivariate statistical analysis was conducted for classification.Results All the four kinds of active components in Chinese yam and its adulterants were detected,and the total polysaccharide content was the highest,accounting for about 20%of the weight,and accounting for more than 90%of the four components.The classification of Chinese yam and its adulterants could be realized based on the contents of four components,and the content of total polyphenols contributed the most to the classi-fication.There was no significant quality difference among the main producing areas of Chinese yam.Conclusion The meth-od established in this experiment is accurate and reliable.By determining the contents of 4 kinds of active components,the classification of Chinese yam and its adulterants can be realized,which provides scientific basis for quality control of Chinese yam based on functional substance and further development and utilization of Chinese yam planting resources.

Dioscorea oppositaTotal polysaccharidesTotal saponinsTotal flavonoidsTotal polyphenolsPrincipal component analysis

金一宝、佟月、陈佳佳、王麓、殷果

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深圳市药品检验研究院,国家药品监督管理局中药质量研究与评价重点实验室,深圳市药品质量标准研究重点实验室,广东深圳 518057

沈阳药科大学,辽宁沈阳 110016

广州中医药大学,广东广州 510006

山药 总多糖 总皂苷 总黄酮 总多酚 主成分分析

深圳市科创委可持续发展项目

KCXFZ20201221173005015

2024

药学研究
山东省药品检验所 山东省药学会

药学研究

CSTPCD
影响因子:0.653
ISSN:2095-5375
年,卷(期):2024.43(3)
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